Journal:
Article Title: Enhanced expression of transient receptor potential channels in idiopathic pulmonary arterial hypertension
doi: 10.1073/pnas.0405908101
Figure Lengend Snippet: TRPC6 mRNA and protein expression is increased in pulmonary tissues from IPAH patients. (A) Representative and summarized (NPH, n = 3; SPH, n = 8; IPAH, n = 3; normalized to α-actin) TRPC6 protein expression in SPH, NPH, and IPAH lung tissues and their α-actin controls. (B) Representative and summarized (n = 3 for each) data showing TRPC6 mRNA products in isolated NPH-, SPH-, or IPAH-PASMC. M, 100-bp DNA ladder. (C) Representative and summarized (n = 5 for each) TRPC6 protein expression in isolated PASMC from NPH, SPH, and IPAH patients. (D) Representative (Top, ×40; Middle, ×100) and summarized immunofluorescence staining of FITC-conjugated TRPC6 (Bottom) in isolated NPH-(n = 11), SPH (n = 21), and IPAH-(n = 36) PASMC. (Scale bars, 20 μm.) ***, P < 0.001 vs. NPH and/or SPH.
Article Snippet: Human PASMC from normal subjects (NPH) were purchased from Cambrex, cultured at 37°C in SMGM, and used at the fourth to sixth passage.
Techniques: Expressing, Isolation, Immunofluorescence, Staining